Jackie 'O' Henderson has resigned from her role on the Kyle and Jackie O Show on KIIS FM, ending one of Australian radio's longest-running breakfast partnerships. Her departure came after she declared she could no longer work alongside co-host Kyle Sandilands, who was suspended by the network for 14 days over serious misconduct during a live broadcast. Australian Radio Network has confirmed it will permanently cancel the program.

Henderson, who had been co-hosting the Sydney-based breakfast show with Sandilands for more than two decades, is understood to have been on a multimillion-dollar contract with ARN. Sandilands' 14-day suspension was imposed after an on-air incident that ARN classified as a serious breach of workplace standards. The network has not disclosed the specific details of the misconduct but confirmed an internal investigation preceded the decision.

The Kyle and Jackie O Show had been a dominant force in Australian commercial radio since the pair first teamed up in 2000 on Sydney's Hot 30 Countdown. Their move to KIIS 106.5 in 2014 cemented the program as the top-rating FM breakfast show in the Sydney market for years. ARN invested heavily in the duo, including a high-profile and ultimately unsuccessful attempt to launch a Melbourne simulcast in 2024.

Industry figures have expressed shock at the sudden collapse of the partnership, with several describing it as a seismic moment for Australian media. Henderson is yet to make a detailed public statement beyond confirming her resignation, while Sandilands has not commented since his suspension was announced. ARN chief executive Ciaran Davis thanked both hosts for their contributions but said the network's decision to end the show was final.

The cancellation leaves a significant gap in KIIS FM's Sydney breakfast lineup, and ARN will need to move quickly to announce a replacement program before advertising commitments are affected. Henderson is expected to attract offers from rival networks and streaming platforms, given her strong audience following and brand partnerships. The fallout is also likely to reignite debate about workplace conduct standards in the Australian broadcasting industry.